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[Preparation and identification of monoclonal antibody against repression and activation protein 1].
Published: 03/06/2015, Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology
Objective
To prepare and identify monoclonal antibody (mAb) against human telomere-associated repression and activation protein 1 (Rap1).
Methods
BALB/c mice were immunized with recombinant Rap1 protein. The spleen cells of the immunized mice were fused with Sp2/0 cells. The positive clones were confirmed and selected by indirect ELISA for titer determination and specificity identification.
Results
One hybridoma cell strain secreting specific mAb against Rap1 was obtained, namely 6F11. ELISA showed that the titer of the ascites was 1:10 000. Western blotting, immunoprecipitation and immunofluorescence experiments demonstrated that the mAb could specifically recognize and bind Rap1.
Conclusion
We have prepared mAb against human telomere-associated protein Rap1. The Rap1 mAb has a good binding ability and specificity.
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